Composite

Part:BBa_K101009:Design

Designed by: Kristen J Lindblad   Group: iGEM08_Minnesota   (2008-07-28)


Dual-Repressed Promoter for p22 Mnt and TetR with RBS (med strength)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 42
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The dually-repressed promoter contains a TetR operator site derived from the pTet promoter of E. coli upstream of the -35 polymerase binding site, and a P22 Mnt operator site between the -35 and -10 polymerase binding sites. The -35 and -10 binding site were derived from the pTet promoter. For information concerning the RBS design, please see part BBa_B0032.


Source

This part combines BBa_K101000, the dually-repressed promoter, with BBa_B0032, a medium strength RBS with good characterization and experience by other registry users.

References